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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Substrates
TMB ELISA Substrate (Highest Sensitivity)
TMB ELISA Substrate (Highest Sensitivity) from Abcam
Be the first to write a review!
Citations:
(13)
Supplier Page
Supplier Page from
Abcam for
TMB ELISA Substrate (Highest Sensitivity)
Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
TMB ELISA Substrate (Highest Sensitivity)
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab171522
Quantity
100 ML, 1000 ML
Applications
ELISA
Target
ELISA substrate
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (13)
(1)
An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol
iScience
October 20, 2023
Yecan Wang, Hiroshi Murakami, Toshihiro Kasama, Shigenobu Mitsuzawa, Satoru Shinkawa, Ryo Miyake, Madoka Takai
50-24-8 Progesterone,FUJIFILM Corp.,CAS RN®: 57-83-0 "3,3′,5,5′-tetramethylbenzidine (TMB) substrate",
Abcam
Co.,
ab171522
"Mucin, from porcine stomach",FUJIFILM Corp.,CAS RN®: 84082-64-4 ,, Critical commercial
assays
,Critical
(2)
Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge
Biomaterials
January 1, 2023
Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, Xu C, Sui C, Ou Y, Liu C,
et al
.
incubated for 40 min at 37 °C. Plates were then washed three-times and added with peroxidase substrate (
Ab171522
,
Abcam
, UK), the reaction was terminated by stop solution (
Ab171529
,
Abcam
, UK) and the absorbance at
(3)
Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses
Proceedings of the National Academy of Sciences of the United States of America
August 9, 2022
Wang Y, Feswick A, Apostolou V, Petkov PM, Moser EK, Tibbetts SA.
1091-05
; IgG2c:
1078-05
; and
IgG3
:
1101-05
). After a final three washes, TMB ELISA Substrate (
Abcam
,
ab171522
) was added to detect HRP enzymatic activity. The reactions were then quenched by the addition of Stop
(4)
Stability of SARS-CoV-2-Encoded Proteins and Their Antibody Levels Correlate with Interleukin 6 in COVID-19 Patients
mSystems
June 28, 2022
Li W, Kitsios GD, Bain W, Wang C, Li T, Fanning KV, Deshpande R, Qin X, Morris A, Lee JS,
et al
.
ab76949
) and 3,3′,5,5′-tetramethylbenzidine (
TMB) ELISA substrate (highest sensitivity
) (catalog number
ab171522
) were purchased from
Abcam
(Cambridge, MA). A
goat anti-mouse IgG
-horseradish peroxidase (HRP) conjugate
(5)
CD164 is a host factor for lymphocytic choriomeningitis virus entry
Proceedings of the National Academy of Sciences of the United States of America
March 8, 2022
Bakkers MJG, Moon-Walker A, Herlo R, Brusic V, Stubbs SH, Hastie KM, Saphire EO, Kirchhausen TL, Whelan SPJ.
respective pH. TMB (3,3′,5,5′-tetramethylbenzidine)
ELISA
Substrate (Highest
Sensitivity
) (
Abcam
No.
ab171522
) was added, followed by equal volumes of
650 nm Stop Solution for TMB Substrate
(
Abcam
No.
ab171531
).
(6)
Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation
Communications biology
February 23, 2022
Pinkaew D, Martinez-Hackert E, Jia W, King MD, Miao F, Enger NR, Silakit R, Ramana K, Chen SY, Fujise K.
gentle shaking. Finally, after extensive washing, we added TMB substrate solution (
Abcam
, Catalog #:
ab171522
) to each well, incubated the plate in the dark for 3 min at room temperature with gentle shaking, added
(7)
Exercise hormone irisin is a critical regulator of cognitive function
Nature Metabolism
August 20, 2021
Mohammad R. Islam, Sophia Valaris, Michael F. Young, Erin B. Haley, Renhao Luo, Sabrina F. Bond, Sofia Mazuera, Robert R. Kitchen, Barbara J. Caldarone, Luis E. B. Bettio,
et al.
secondary antibody for 30 min following 4X PBST wash. 3,3’,5,5’-Tetramethylbenzidine (TMB) chromogen (
ab171522
,
Abcam
) was used as detection systems, and finally, the absorbance was measured at 450nm after adding
(8)
Systemic Treatment of Fabry Disease Using a Novel AAV9 Vector Expressing α-Galactosidase A
Molecular Therapy - Methods & Clinical Development
March 12, 2021
Maria Grazia Biferi, Mathilde Cohen-Tannoudji, Andrea GarcÃa-Silva, Olga Souto-RodrÃguez, Irene Viéitez-González, Beatriz San-Millán-Tejado, Andrea Fernández-Carrera, Tania Pérez-Márquez, Susana Teijeira-Bautista, Soraya Barrera,
et al.
Samples were incubated 1 h at room temperature and then visualized using the BioFX TMB/Stop solution (#
ab171522
;
Abcam
). The reaction was halted with 1 vol of HCl 0.1 N. Absorbance was measured at 450 nm. IgG concentrations
(9)
Bamboo Shark as a Small Animal Model for Single Domain Antibody Production
Frontiers in bioengineering and biotechnology
January 1, 2021
Wei L, Wang M, Xiang H, Jiang Y, Gong J, Su D, Al Azad MAR, Dong H, Feng L, Wu J,
et al
.
secondary antibody and then incubated at RM for 1 h.
ELISA
was developed with TMB substrate (
Abcam
,
ab171522
) and then read at 450 nm. Three
shark
individuals in each
immunization
program were involved in the
(10)
The novel llama-human chimeric antibody has potent effect in lowering LDL-c levels in hPCSK9 transgenic rats
Clinical and translational medicine
February 13, 2020
Li X, Wang M, Zhang X, Liu C, Xiang H, Huang M, Ma Y, Gao X, Jiang L, Liu X,
et al
.
tetramethylbenzidine was added and the plate was incubated in the dark at 25 °C for 10 min (
Abcam
, UK, Cat#
ab171522
). The reaction was stopped by 50 μL 1 M sulfuric acid. The OD450 absorbance was measured by a microplate
(11)
Differential recognition of peptides within feline coronavirus polyprotein 1 ab by sera from healthy cats and cats with feline infectious peritonitis
Virology
June 1, 2019
Anastasia Chernyavtseva, Nick J. Cave, John S. Munday, Magdalena Dunowska
temperature. After five washes with CovaBuffer, 100 μL
TMB ELISA Substrate (Highest Sensitivity
) (
Abcam
,
ab171522
) was added to well and the plate was incubated at 37 °C for 10 min. The reaction was stopped by the
(12)
Synthesis of multivalent glycopeptide conjugates that mimic an HIV epitope
Tetrahedron
October 6, 2016
Jennifer K. Bailey, Dung N. Nguyen, Satoru Horiya, Isaac J. Krauss
washes, the wells were developed by adding 60 µL of 3,3’,5,5’-tetramethylbenzidine (TMB solution,
Abcam
Ab171522
) for 2 min. The reaction was stopped by adding 60 µL of 1M sulfuric acid and absorbance was measured
(13)
[Integrated optical sensor for rapid analysis of biological samples]
Orvosi Hetilap
December 27, 2015
Anna Mathesz, Sándor Valkai, Orsolya Sipos, Balázs Stercz, Béla Kocsis, Dóra Szabó, András Dér
000-szeres hígításban. Ismételt
Tween 20
-as mosás után hozzáadtuk a szubsztrátot (
Abcam
, Cat. No.:
ab171522
), majd 25 perc elteltével 2N kénsavval (R&D Systems, Cat. No.:
DY994
) megállítottuk a reakciót, és 450
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Reviews
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
TMB ELISA Substrate (Highest Sensitivity) from Abcam
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